Streak plate technique-

The inoculum is streaked or spread over the surface of a solid medium. The streaking process accomplishes a thinking out of the population. There is no uniform distribution of the organism. Where they are thickly populated, they will grow in mass, but where only few are present, they develop distinct colonies. These isoalated colonies are the progenies of single cells and whether they are so may be verified from microscopic examination. Pure culture may be recurred by the transfer of a part of such colonies to a container with suitable culture medium.